Review





Similar Products

actin a3853  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc actin a3853
Actin A3853, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actin a3853/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
actin a3853 - by Bioz Stars, 2026-06
97/100 stars
  Buy from Supplier
β actin a3853  (Merck & Co)
86
Merck & Co β actin a3853
β Actin A3853, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β actin a3853/product/Merck & Co
Average 86 stars, based on 1 article reviews
β actin a3853 - by Bioz Stars, 2026-06
86/100 stars
  Buy from Supplier
mouse anti-β-actin a3853  (Millipore)
90
Millipore mouse anti-β-actin a3853
Mouse Anti β Actin A3853, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-β-actin a3853/product/Millipore
Average 90 stars, based on 1 article reviews
mouse anti-β-actin a3853 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
β‐actin (a3853)  (Millipore)
90
Millipore β‐actin (a3853)
β‐Actin (A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β‐actin (a3853)/product/Millipore
Average 90 stars, based on 1 article reviews
β‐actin (a3853) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
anti-β-actin antibody (#a3853)  (Millipore)
90
Millipore anti-β-actin antibody (#a3853)
Anti β Actin Antibody (#A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-β-actin antibody (#a3853)/product/Millipore
Average 90 stars, based on 1 article reviews
anti-β-actin antibody (#a3853) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
actin ac-40 (a3853, 1:1000)  (Merck & Co)
90
Merck & Co actin ac-40 (a3853, 1:1000)
Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with <t>Ac-40</t> actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.
Actin Ac 40 (A3853, 1:1000), supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actin ac-40 (a3853, 1:1000)/product/Merck & Co
Average 90 stars, based on 1 article reviews
actin ac-40 (a3853, 1:1000) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
anti-actin mouse antibody a3853  (Millipore)
90
Millipore anti-actin mouse antibody a3853
Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with <t>Ac-40</t> actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.
Anti Actin Mouse Antibody A3853, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-actin mouse antibody a3853/product/Millipore
Average 90 stars, based on 1 article reviews
anti-actin mouse antibody a3853 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
β-actin (cat# a3853) antibody, bacterial lps) from e. coli o111:b4 (cat# l4391, lot 115m4090v)  (Millipore)
90
Millipore β-actin (cat# a3853) antibody, bacterial lps) from e. coli o111:b4 (cat# l4391, lot 115m4090v)
Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with <t>Ac-40</t> actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.
β Actin (Cat# A3853) Antibody, Bacterial Lps) From E. Coli O111:B4 (Cat# L4391, Lot 115m4090v), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β-actin (cat# a3853) antibody, bacterial lps) from e. coli o111:b4 (cat# l4391, lot 115m4090v)/product/Millipore
Average 90 stars, based on 1 article reviews
β-actin (cat# a3853) antibody, bacterial lps) from e. coli o111:b4 (cat# l4391, lot 115m4090v) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
actin a3853 antibody  (Millipore)
90
Millipore actin a3853 antibody
Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with <t>Ac-40</t> actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.
Actin A3853 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actin a3853 antibody/product/Millipore
Average 90 stars, based on 1 article reviews
actin a3853 antibody - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
β-actin (a3853)  (Millipore)
90
Millipore β-actin (a3853)
Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with <t>Ac-40</t> actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.
β Actin (A3853), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β-actin (a3853)/product/Millipore
Average 90 stars, based on 1 article reviews
β-actin (a3853) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with Ac-40 actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.

Journal: The Journal of Biological Chemistry

Article Title: Actin associates with actively elongating genes and binds directly to the Cdk9 subunit of P-TEFb

doi: 10.1016/j.jbc.2024.105698

Figure Lengend Snippet: Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with Ac-40 actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.

Article Snippet: The following antibodies were from Merck: Actin Ac-40 (A3853, 1:1000), Actin Ac-74 (A2228), HRP-conjugated polyHistidine (His-HRP, A7058, 1:5000), HRP-conjugated anti-goat IgG (A9452, 1:5000), HRP-conjugated anti-Flag M2 (A8592, 1:5000), and HRP-conjugated anti-HA (HA-7) (H6533, 1:5000).

Techniques: Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Construct, Expressing, Binding Assay, Fluorescence, Labeling